Randhir Singh
M.M. University, India
Title: Nephroprotective effect of Dillenia indica L. in STZ-induced diabetic rats via inhibition of oxidative stress
Biography
Biography: Randhir Singh
Abstract
Etiology of diabetic nephropathy is multifactorial with many pathogenetic mechanisms. Many plant products are being used traditionally in the management of diabetes and its complications. Our study was aimed to evaluate the potential of hydro-alcohol extract (DHA) of Dillenia indica L. (Family: Dilleniaceae) in treatment of diabetic nephropathy by targeting renal markers and oxidative stress. DHA was evaluated for its inhibitory activity against formation of AGEs by using bovine serum albumin, sorbitol accumulation in human blood and aldose reductase in rat kidney. Diabetic nephropathy was assessed by determining serum glucose, insulin, renal parameters (urea, uric acid, creatinine level) and tissue histological examination. Tissue antioxidant enzymes (SOD, CAT and GSH) and LPO levels were measured along with the formation of AGEs in kidney to assess the effect of DHA in ameliorating oxidative stress. DHA showed significant inhibition against sorbitol accumulation and aldose reductase activity. DHA also produced significant attenuation in the glycemic status, renal parameter, lipid profile and level of antioxidant enzymes proving efficacy in diabetic nephropathy. Moreover, DHA has also produced significant reduction in the formation of AGEs in vitro as well as in kidneys. The results obtained from the study indicate the significant potential of D. indica as a possible therapeutic agent against diabetic nephropathy