Edyta Gendaszewska-Darmach
Lodz University of Technology
Poland
Title: A comparative evaluation of phosphorothioate and phosphodiester analogues of lysophosphatydylcholine toward murine β pancreatic cells: in vitro studies
Biography
Biography: Edyta Gendaszewska-Darmach
Abstract
Based on the results of research conducted recently, lysophosphatidylcholine (LPC) has been observed to be not only a structural component of cellular membranes but also a biologically active molecule influencing regulation of metabolic diseases, such as obesity and diabetes. Much attention has been paid to the fact that LPC causes an increase in glucose-stimulated insulin secretion from β pancreatic cells. To address the need of further characterization of various LPC species with regard to diversity of their structures as well as longer biological half-lives, we have recently described the chemical synthesis of new sulfur analogues of LPC with well-defined fatty acid residues. In order to prevent possible 1→2 acyl migration in LPC analogues, the oxygen atom in position 2 of glycerol was protected by methylation. A series of phosphorothioate and phosphorodithioate derivatives of 2-OMe-LPC bearing five different fatty acid residues both saturated (12:0, 14:0, 16:0, 18:0) and unsaturated (18:1) were prepared. Preliminary studies towards pancreatic βTC-3 cells proved that even a slight difference in chemical structure of a stimulus may result in significant changes in the exerted biological effects
