Day 1 :
Time : 10:00-10:30 AM
Ravinder J Singh is currently the Director of the Endocrinology Laboratory at the Mayo Clinic. One of his areas of expertise is application of liquid chromatographyrntandem mass spectrometric (LC-MS/MS) to clinical laboratory analysis. He has published his work on originally developed methods including such analytes asrncortisol, synthetic corticosteroids, metanephrines, estradiol, vitamin D, and urine albumin amongst others. He is also the Co-PI of 3 grants. He is Co-director of thernICL lab which supports the Human research protocols. He was recently granted a Novel methodology CTSA award for “Development of Robust LC-MS/MS BasedrnMethods for the Absolute Quantification of Proteins and Peptides.” And he was recently awarded AACC Outstanding Speaker Award, as well as MSACL LeadershiprnAward for Mass Spectometry Applications to the clinical lab.
The quality and standardization of HbA1c methods in the clinical laboratories will be discussed. The differences in variousrnmethods for mesuring HbA1c will be highlighted. The gold standard methodolgy and challenges in standardization of therntesting will be presented. Interferences and specificty related to various insulin assays will also be discussed. The quality of thernPOCT for glucose in real life will also be highlighted. How use of artificial pancreas will impact the management of diabetesrnwill be part of the discussion as well
Walter Sisulu University
Keynote: Biomarkers of oxidative stress and antioxidant status in type 2 diabetic patients – A study among African diabetic patients on treatment
Time : 10:30-11:00
George G completed her PhD in 1980 from Central Food Technological Research Institute, Mysore. India. Currently she is working as a Professor and Head ofrnthe Division of Medical Biochemistry, Faculty of Health Sciences at Walter Sisulu University, South Africa. Many Post-graduate students graduated under herrnsupervision and she has several publications and presentations to her credit.
The metabolic derangement in diabetes mellitus leads to increased oxidative stress in the body and consequent developmentrnof the disabling chronic complications. The aim of the present study was to evaluate oxidative stress parameters and totalrnantioxidant status in type 2 diabetic patients in the region and to compare it with that of healthy non diabetic controls. 57rntype 2 diabetic patients and 41 normal healthy controls consented and participated in the study. Fasting blood glucose (FBS),rnGlycosylated haemoglobin and lipid profile were assayed by routine laboratory methods. Serum total antioxidant capacityrn(TAO), thiobarbituric acid reactive substances (TBARS) and the antioxidant enzyme superoxide dismutase (SOD) and oxidizedrnLDL levels were measured using standard commercial reagent kits employing ELISA technique. The results expressed as meanrn± SD. The results indicated statistically significant increases in FBS (p<0.000), HbA1c (p<0.000), triglycerides (p<0.031),rnTBARS (p<0.004) and oxidized LDL (p<0.022) in type 2 diabetic patients as compared to control population, whereasrntotal antioxidant activity (p<0.010) and HDL cholesterol (p<0.008) were significantly decreased in diabetic patients. Totalrncholesterol, LDL cholesterol and SOD enzyme activity did not show any significant changes among both groups. Oxidativernstress indicators showed positive correlation with FBS and HbA1c while there was significant negative correlation with TAOrnand HDL cholesterol. The above results warrant intervention through a diet rich in antioxidants, antioxidant vitamins and lifernstyle modifications to prevent the debilitating complications of diabetes in these patients.
Madurai Kamaraj University
Keynote: Role of microRNAs in diabetic heart
Time : 11:20-11:50
Subbiah Ramasamy has completed his PhD in Molecular Cardiology from Madurai Kamaraj University and Post-doctoral research at University of Temple,rnPhiladelphia and University of Alabama at Birmingham, USA. He has joined Madurai Kamaraj University as an Assistant Professor in 2010. He has published morernthan 20 papers in reputed journals.
Diabetes is one of the most common chronic disorders in the world population. Numerous and complex factors includingrnvarious genetic and physiological changes can lead to develop two types of diabetes: Type-1 diabetes (T1D) is due to selfdestructionrnof the insulin producing β-cells/Type-2 diabetes (T2D) is caused by defects in insulin production in the pancreas.rnBoth types of diabetes develop serious secondary complications, such as microvascular complications, oxidative stress, footrncomplications related to peripheral, neuropathy, peripheral vascular disease and cardiovascular disease. However, the majorrnmolecular mechanisms of pathogenesis of diabetic with cardiac complications remain obscure. With the recent discovery ofrnMicroRNAs (miRNAs), these small non-coding RNAs have been implicated as new players in the pathogenesis of diabetes andrndiabetes-associated cardiac complications. Significantly, the gene profiling techniques has been widely used as early diagnosticrnand prognostic biomarker especially in the area of diabetic cardiac complication research.