7th Indo Global Diabetes Summit and Medicare Expo
Walter Sisulu University
Title: Biomarkers of oxidative stress and antioxidant status in type 2 diabetic patients – A study among African diabetic patients on treatment
Biography: Grace George
The metabolic derangement in diabetes mellitus leads to increased oxidative stress in the body and consequent developmentrnof the disabling chronic complications. The aim of the present study was to evaluate oxidative stress parameters and totalrnantioxidant status in type 2 diabetic patients in the region and to compare it with that of healthy non diabetic controls. 57rntype 2 diabetic patients and 41 normal healthy controls consented and participated in the study. Fasting blood glucose (FBS),rnGlycosylated haemoglobin and lipid profile were assayed by routine laboratory methods. Serum total antioxidant capacityrn(TAO), thiobarbituric acid reactive substances (TBARS) and the antioxidant enzyme superoxide dismutase (SOD) and oxidizedrnLDL levels were measured using standard commercial reagent kits employing ELISA technique. The results expressed as meanrn± SD. The results indicated statistically significant increases in FBS (p<0.000), HbA1c (p<0.000), triglycerides (p<0.031),rnTBARS (p<0.004) and oxidized LDL (p<0.022) in type 2 diabetic patients as compared to control population, whereasrntotal antioxidant activity (p<0.010) and HDL cholesterol (p<0.008) were significantly decreased in diabetic patients. Totalrncholesterol, LDL cholesterol and SOD enzyme activity did not show any significant changes among both groups. Oxidativernstress indicators showed positive correlation with FBS and HbA1c while there was significant negative correlation with TAOrnand HDL cholesterol. The above results warrant intervention through a diet rich in antioxidants, antioxidant vitamins and lifernstyle modifications to prevent the debilitating complications of diabetes in these patients.
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