Purabi Sarkar
Gauhati University, India
Title: Dietary antioxidants induces differential gene expression of oxidative stress pathway genes in l6 cell line under diabetic condition
Biography
Biography: Purabi Sarkar
Abstract
Type 2 diabetes (T2D) is the most prevalent and serious metabolic disease all over the world. Oxidative stress caused by increased generation of reactive oxygen species (ROS) under hyperglycemia may contribute to the development of T2D. Therefore, cells have antioxidant networks to scavenge excessively produced ROS and antioxidants neutralize these ROS and decrease oxidative stress. The present study was designed to evaluate and compare the effect on oxidative stress pathway genes upon supplementation of antioxidants Resveratrol and Estragole in L6 cell line to combat diabetic stress conditions by qPCR system. In this study the results show up- regulation of GST and SOD gene in L6 cell lines treated with Antioxidant alone ,HNE+Antioxidant, Antioxidant+Insulin and HNE+Antioxidant+Insulin combinations. When compared with the control, Resveratrol+ Insulin treatment shows 2.48 folds up-regulation in GST gene and 2.28 folds up-regulation in SOD gene as compared to other treatment set. Similarly, Estragole also up-regulate GST expression by 2.15 folds and SOD expression by 2.34 folds in the Estragole+Insulin treatment set. In case of GPx gene, Estragole up-regulate by 0.60 folds in cells treated only with Estragole and Resveratrol show down-regulation of 2.04 folds in the HNE+Antioxidant treatment set. Whereas, CAT gene down-regulate by 3.22 folds in HNE+Antioxidant+Insulin treatment set for Estragole and Resveratrol up-regulate by 0.94 folds in only Resveratrol treatment. The study demonstrates that antioxidants Resveratrol and Estragole may be effective in combating ROS scavenging in patients with T2D. We have found Insulin alone can combat diabetes but its combination with dietary antioxidant perform better and are more effective.