7th Indo Global Diabetes Summit and Medicare Expo
Teerthankar Mahaveer University
Title: In vitro toxicity screening of polyphyto combination using Caco-2-cell line
Biography: B. Abhishek
The aim of the research work is polyphyto combination compression of different antilithiogenic agent and to screen its toxicity by Caco-2-cell line. Polyphyto combination was prepared by geometrical dilution method; it was subjected for aqueous and alcoholic extract. The formulation and extract were screened for its initial in vitro toxicity study using Caco-2-cell line by 3-(4, 5 dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay method for aqueous extract of formulation. It showed nontoxic to Caco-2-cell line, even at high dose, that is, 1000 µg/ml, whereas aqueous extract (FH1) exhibited moderate toxicity CTC 50 value 875±35.3 µg, FH1 crude formulation showed significant toxicity against Caco-2-cells with low concentration with CTC 50 value 382.75 based on this result the conclusion was drown that the formulation FH2 and aqueous extract of FH1 is devoid of toxicity. The cells were dissociated with TPVG solution (0.2% trypsin, 0.02% EDTA, 0.05% glucose in PBS).