7^th Indo Global Diabetes Summit and Medicare Expo

Biography

Biography: Monisha Benerjee

Abstract

The onset and progression of Type 2 diabetes mellitus (T2DM) result due to an alteration in cytokine levels, prostaglandin E2 (PGE2) and cyclooxygenases (COX). COX is responsible for PGE2 production and is regulated by inflammatory cytokines viz. IL-4, IL-13, and IL-10 while pro-inflammatory cytokines viz. IL-6 and TNF-α are differentially regulated by PGE2 and COX. Our objective was to study the association of selected gene variants viz. IL-4, IL-6, IL-10, TNF-α, COX2 and COX expression in T2DM. Blood samples from 1,157 subjects (717 controls and 440 cases) were collected for genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) after ethical approval and individual consent. Serum IL-10 level was measured by Enzyme Linked Immunosorbent Assay (ELISA). Genotypic and haplotypic analyses were performed by SPSS (v-21.0) and SHEsis (v-online). Expression analysis was performed by real time-PCR and data were analyzed using Prism software (v-5.0). VNTR (70bp) in IL-4-Intron3, ‘GG’ of IL-6-174G/C, TC’ genotype of IL-10-819T/C, ‘GG’ of IL-10-1082A/G, and ‘GG’ of COX2+1195T/C showed significant association with T2DM (P<0.01). Individuals with haplotype ‘TG’ of IL-10-819T/C and -1082A/G, ‘CG’ of IL-6-174G/C and -597C/G showed 1-3 times higher risk of developing T2DM. Results showed significant increase of serum IL-10 levels in T2DM cases (P<0.033). COX1 and COX2 genes showed higher expression in T2DM as compared to controls (1.04, 1.034 folds respectively). ‘TT’ genotype of COX2+1195T/C showed 1.024 folds higher mRNA expression. Cytokine gene variants might help in revealing individual disease risk and understand the relation between cytokines and COX expression in T2DM