7th Indo Global Diabetes Summit and Medicare Expo
Title: Development of Innovative technologies to detect long-term diabetes using Human toenail by Vibrational spectroscopy
Biography: Mohammed Farhan Katheem
Background: Diabetes mellitus is a fastest growing non communicable disease in India. The bio-molecule, protein is mainly affected by diabetic condition due to presence of reactive functional groups results in glycation and aggregation. The human nail plate is act as shield and it gives protection to human body and composed of hard α – keratin, which is the substance forming stratum corneum. Acceptable differences in infrared (IR) spectra of Diabetic (D), Prone to Diabetic (PD) and Non-Diabetes (ND) human toenail specimens were investigated in this study.
Methods: Both male and female subjects’ toenail specimens with different age group were recruited. The different groups’ nails were studied by using PerkinElmer Fourier Transform Infra Red (FTIR) spectrometer data were correlated with clinical investigations.
Results: The toe nail of D & ND were studied extensively had shown amide I, II and III bands are observed with slight shifting with reference to age groups and duration of diabetes. The amide III bands are present in both D & ND but the alkylthiolated bands still only present in diabetic patients. Clinical results have shown difference in all three group subjects.
Conclusion: The protein in the nails of D contains α-helical structure, including the presence of amide II bonds where as it is absent in ND. In D & ND toe nails are showing more or less same results for amide I, II & III. The alkyl thiolation is unique in chronic diabetic patients in toe nail too. It is helpful in screening diabetes for long-term basis in chronic diabetic patients with ref to their toe nail growth. The PD data gave important clues between D & ND groups.