Richa Chaturvedi
Assam University
India
Title: Supplementation of Iron rich drinking water and ascorbic acid improved hemolytic anemia in Wistar rat model
Biography
Biography: Richa Chaturvedi
Abstract
High concentrations of iron in the body can inhibit phagocytosis and transporter proteins. If there is a reduction in the liver iron, it can improve the glucose/insulin axis. This study documents the effect of administration of iron rich water on hemolytic anemia in a Wistar rat’s animal model. Hemolytic anemia was induced intraperitoneally by phenyl hydrazine and diagnosed by blood hemoglobin lowering. After inducing the hemolytic anemia, 24 Wistar rats (n=6 in four groups) were randomly assigned to 1mg/l, 5 mg/l and 10 mg/l ferric oxide iron along with 1mg/l ascorbic acid administered through drinking water, a control group was treated with iron-free water. After 30 days of treatment, the hematological and biochemical parameters, iron levels in liver, spleen and kidney were estimated. A significant increase of serum iron and ferritin and a decrease of TIBC (total iron binding capacity) were observed without changes in other biochemical parameters in the group treated with 5mg/l iron and ascorbic acid. However, in the group treated with 10 mg/ml iron and ascorbic acid, hematological changes with significantly higher values for white blood cell count, glucose, splenic and liver iron content indicates potential toxicity at this supplementation level. The result suggest that the optimum concentration of iron (5 mg/l) and ascorbic acid may improve anemic conditions and may be therapeutically beneficial in the treatment of anemia without any negative impact, while 10 mg/l in drinking water seems to be the threshold for the initiation of toxicity.